Carbapenem-resistant IMP Detection K-Set (Lateral Flow Assay)
Chiyambi cha Zamalonda
Carbapenem-resistant IMP Detection K-Set (Lateral Flow Assay) ndi njira yoyesera ya immunochromatographic yopangidwira kuzindikira kwamtundu wa IMP-carbapenemase m'magulu a bakiteriya.Kuyesaku ndi kuyesa kwa labotale yogwiritsira ntchito mankhwala komwe kungathandize kuzindikira mitundu ya IMP-mtundu wa carbapenem.
Makhalidwe
| Dzina | Carbapenem-resistant IMP Detection K-Set (Lateral Flow Assay) |
| Njira | Lateral Flow Assay |
| Mtundu wachitsanzo | Matenda a tizilombo |
| Kufotokozera | 25 mayeso / zida |
| Nthawi yozindikira | 10-15 min |
| Kuzindikira zinthu | Carbapenem-resistant Enterobacteriaceae (CRE) |
| Mtundu wozindikira | IMP |
| Kukhazikika | K-Set imakhala yokhazikika kwa zaka 2 pa 2 ° C-30 ° C |
Ubwino
- Mwamsanga
Pezani zotsatira mkati mwa mphindi 15, masiku atatu m'mbuyomo kusiyana ndi njira zodziwika bwino - Zosavuta
Zosavuta kugwiritsa ntchito, ogwira ntchito za labotale wamba amatha kugwira ntchito popanda maphunziro - Zolondola
Mkulu tilinazo ndi mwachindunji
Malire odziwika otsika: 0.20 ng/mL
Amatha kuzindikira mitundu ingapo yodziwika bwino ya IMP
- Chotsatira chanzeru
Palibe chifukwa chowerengera, zotsatira zowerengera zowonera - Zachuma
Mankhwala akhoza kunyamulidwa ndi kusungidwa kutentha kwa firiji, kuchepetsa ndalama
Kufunika kwa mayeso a CRE
Pamodzi, Enterobacterales ndi gulu lofala kwambiri la tizilombo toyambitsa matenda omwe amayambitsa matenda okhudzana ndi thanzi.Ma Enterobacterales ena amatha kupanga enzyme yotchedwa carbapenemase yomwe imapangitsa maantibayotiki monga carbapenems, penicillins, ndi cephalosporins kukhala osagwira ntchito.Pachifukwachi, CRE amatchedwa "mabakiteriya owopsa" chifukwa pali maantibayotiki ochepa, ngati alipo, omwe atsala kuti athe kuchiza matenda oyambitsidwa ndi majeremusiwa.
Mabakiteriya ochokera ku banja la Enterobacterales, kuphatikizapo mitundu ya Klebsiella ndi Escherichia coli, amatha kupanga carbapenemase.Carbapenemases nthawi zambiri amapangidwa kuchokera ku majini omwe ali pazinthu zosunthika zomwe zimatha kufalitsa kukana mosavuta kuchokera ku majeremusi kupita ku majeremusi komanso munthu kupita kwa munthu.Komanso chifukwa cha kugwiritsiridwa ntchito molakwika kwa maantibayotiki ndi njira zochepa zomwe zimatengedwa pofuna kupewa kufalikira, vuto la CRE lomwe likuchulukirachulukira likukhala pachiwopsezo cha moyo padziko lonse lapansi.
Nthawi zambiri, kufalikira kwa CRE kumatha kuwongoleredwa ndi:
- Kuyang'anira matenda a CRE
- Patulani odwala omwe ali ndi CRE
- Kuchotsa zida zachipatala zowononga mkati mwa thupi
- Samalani popereka maantibayotiki (makamaka carbapenems)
- Kugwiritsa ntchito njira zoyera zosabala kuti muchepetse kufalikira kwa matenda
- Tsatani ndondomeko yoyeretsa labu
………
Kuzindikira kwa CRE ndikofunikira kwambiri pakuwongolera kufalikira.Poyesa msanga, othandizira azaumoyo amatha kupereka chithandizo choyenera kwa odwala omwe ali ndi CRE, komanso kukwaniritsa kasamalidwe kachipatala.
IMP-mtundu carbapenemase
Carbapenemase imatanthawuza mtundu wa β-lactamase womwe ungathe hydrolyze imipenem kapena meropenem, kuphatikizapo A, B, D mitundu itatu ya michere yomwe imayikidwa ndi Ambler molecular structure.Mwa iwo, Gulu B ndi metallo-β-lactamases (MBLs), kuphatikizapo carbapenemases monga IMP, VIM ndi NDM,.IMP-type carbapenemase, yomwe imadziwikanso kuti imipenemase metallo-beta-lactamase yotulutsa CRE, ndi mtundu wamba wopezeka wa MBLs ndipo imachokera ku subclass 3A.Imatha hydrolyze pafupifupi maantibayotiki onse a β-lactam.
Ntchito
- Onjezani madontho 5 a njira yothetsera mankhwala
- Dikirani mabakiteriya okhala ndi katemera wotayika
- Ikani lupu mu chubu
- Onjezani 50 μL ku chitsime cha S, dikirani kwa mphindi 10-15
- Werengani zotsatira zake
Kuitanitsa Zambiri
| Chitsanzo | Kufotokozera | Kodi katundu |
| CPI-01 | 25 mayeso / zida | CPI-01 |
