Kuzindikira Mwachindunji kwa Ma Viral Antibody

Njira zingapo izi ndi zoyeserera pogwiritsa ntchito ma antigen enieni a virus kuti azindikire ma antibodies mu seramu ya odwala, kuphatikiza kuzindikira ma antibodies a IgM ndi kuyeza kwa ma antibodies a IgG.Ma antibodies a IgM amatha pakatha milungu ingapo, pomwe ma antibodies a IgG amapitilira zaka zambiri.Kukhazikitsa kuzindikirika kwa matenda a virus kumachitika serologically powonetsa kukwera kwa antibody titer ku kachilomboka kapena kuwonetsa ma antibodies a gulu la IgM.Njira zomwe zimagwiritsidwa ntchito ndi monga neutralization (Nt) test, complement fixation (CF) test, hemagglutination inhibition (HI) test, immunofluorescence (IF) test, passive hemagglutination, ndi immunodiffusion.

A. Mayeso a Neutralization

Pa nthawi ya matenda kapena chikhalidwe cha ma cell, kachilomboka kangathe kuletsedwa ndi ma antibody ake enieni ndikutaya infectivity, mtundu uwu wa antibody umatanthauzidwa ngati ma antibody neutralization.Kuyesa kwa Neutralization ndikuzindikira ma anti-neutralization mu seramu ya odwala.

B. Malizitsani Mayesero Okonzekera

Kuyesa kokwanira kokwanira kungagwiritsidwe ntchito kuyang'ana kukhalapo kwa antibody kapena antigen mu seramu ya wodwala.Kuyesaku kumagwiritsa ntchito maselo ofiira a nkhosa (SRBC), anti-SRBC antibody ndi othandizira, pamodzi ndi antigen yeniyeni (ngati ikuyang'ana antibody mu seramu) kapena antibody yeniyeni (ngati ikuyang'ana antigen mu seramu).

C. Hemagglutination Inhibition Assays

Ngati kuchuluka kwa ma virus muzachitsanzo ndi kwakukulu, chitsanzocho chikasakanizidwa ndi ma RBCs, ma virus ndi ma RBCs amapangidwa.Chodabwitsa ichi chimatchedwa hemagglutination.Ngati ma antibodies motsutsana ndi hemagglutinins alipo, hemagglutination idzalephereka.Pa hemagglutination chopinga mayeso, siriyo dilution wa seramu wothira odziwika kuchuluka kwa kachilombo.Pambuyo pa makulitsidwe, ma RBC amawonjezeredwa, ndipo kusakaniza kumasiyidwa kukhala kwa maola angapo.Ngati hemagglutination yaletsedwa, pellet ya RBCs imapanga pansi pa chubu.Ngati hemagglutination sichiletsedwa, filimu yopyapyala imapangidwa.