Kuyambira Januware 2020 mpaka Okutobala 2020, kafukufuku woyembekezera adachitika pachipatala cha Pisa University chomwe chidasindikizidwa pa BMC Microbiology.Goldstream®Bowa (1-3) -β-D-Glucan Test idagwiritsidwa ntchito pozindikira mulingo wa BDG kuchokera ku zitsanzo za BAL.Zotsatira zake zidawerengedwa ndi aFull Automatic Kinetic Tube Reader IGL-200kuchokera ku Era Biology.Kafukufukuyu akuwonetsa kuti BDG iyenera kuganiziridwa chifukwa cha mtengo wake wolosera kwambiri.Ndipo zidakhala zothandiza pakuletsa kuzindikirika kwa PCP kwa odwala onse owongolera.
Mbiri:
The panopa matenda golide muyezo waPneumocystis jiroveciiimayimiridwa ndi mawonekedwe ang'onoang'ono a bowa kuchokera ku zitsanzo za kupuma, monga bronchoalveolar-lavage fluid, kutanthauza "kutsimikiziridwa"P. jiroveciichibayo, pamene qPCR imalola kufotokoza "chotheka" matenda, chifukwa sichingathe kusankha matenda kuchokera ku colonization.Komabe, njira za mamolekyu, monga mapeto a PCR ndi qPCR, ndi zachangu, zosavuta kuchita ndi kumasulira, motero zimalola kuti ma labotale abwererenso kwa dokotala deta yothandiza ya tizilombo toyambitsa matenda mu nthawi yochepa.Kafukufuku wapano akufuna kufananiza ma microscopy ndi ma assay a maselo ndi ma beta-D-glucan diagnostic amadzimadzi a bronchoalveolar-lavage kuchokera kwa odwala omwe akuwakayikira.Pneumocystis jiroveciichibayo.Bronchoalveolar-lavage fluid kuchokera ku maphunziro khumi ndi asanu ndi atatu omwe ali pachiwopsezo chachikulu komanso maphunziro anayi oyipa adakumana ndi Grocott-Gomori's methenamine silver-staining, end-point PCR, RT-PCR, ndi beta-D-glucan assay.
Zotsatira:
Zitsanzo zonse zowoneka bwino za bronchoalveolar-lavage (50%) zidapangitsanso PCR yomaliza ndi nthawi yeniyeni ndi zonse, koma ziwiri, zidakhala zabwino ndi kuchuluka kwa beta-D-glucan.Mapeto a PCR ndi RT-PCR adazindikira 10 (55%) ndi 11 (61%) mwa zitsanzo za 18, motero, kusonyeza kukhudzidwa kowonjezereka poyerekeza ndi microscopy.Ma RT-PCR onse okhala ndi Ct<27 adatsimikiziridwa pang'ono, pomwe zitsanzo zokhala ndi Ct≥27 sizinali.
Mapeto:
Ntchito yathu ikuwonetsa kufunikira kokonzanso ndikutanthauziranso gawo lazachipatala pazachipatala, mongaP. jiroveciimatenda, omwe ndi osowa komanso owopsa komanso omwe akupita patsogolo mwachangu omwe amakhudza omwe ali ndi chitetezo chamthupi omwe angapindule kwambiri ndikuwazindikira mwachangu.Odwala osankhidwa bwino, molingana ndi njira zophatikizira, zotsatira zoyipa ndi njira zama cell zitha kutsatiridwaP. jiroveciichibayo.
Franconi I, Leonildi A, Erra G, et al.Kuyerekeza njira zosiyanasiyana za microbiological za matenda a Pneumocystis jirovecii chibayo pa bronchoalveolar-lavage fluid.BMC Microbiol.2022;22(1):143.Lofalitsidwa 2022 Meyi 21. doi:10.1186/s12866-022-02559-1
Nthawi yotumiza: Jun-14-2022